Invitrogen™ One Shot™ TOP10/P3 Chemically Competent E. coli

Description

One Shot TOP10/P3 Chemically Competent E. coli cells are designed for vector transformation encoding the synthetic supF gene (tyrosine tRNA suppressor) and allow high-quality pCDM8, pcDNA1.1 plasmid, or any other supF-containing vector preparation.

One Shot TOP10/P3 Chemically Competent E. coli cells are designed for vector transformation encoding the synthetic supF gene (tyrosine tRNA suppressor) and allow high-quality pCDM8, pcDNA1.1 plasmid, or any other supF-containing vector preparation.

One Shot TOP10/P3 strain features
• P3 plasmid for selection of supF-containing plasmids
• Mutated endA1 gene for increased quality of plasmid DNA preparations
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
• lacZΔM15 for blue/white color screening of recombinant clones

P3 Plasmid
The P3 plasmid is a low-copy-number, 60 kb plasmid that carries kanamycin, tetracycline and ampicillin resistance markers. The kanamycin gene is fully active and is used to select for cells carrying P3. Tetracycline and ampicillin genes carry amber mutations which render the genes inactive during normal growth and replication of the bacteria. Upon transformation of a vector carrying the suppressor F gene, the amber mutations in the tetracycline and ampicillin genes on the P3 plasmid are suppressed and the E. coli are resistant to these antibiotics.

Genotype
F^- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG P3: KanR AmpR (am) TetR (am) 

Find the strain and format that you need
• TOP10 is offered in chemically competent and electrocompetent cell formats.
• Choose MultiShot competent cells for high-throughput cloning applications.

Specifications

• Product Type: Chemically Competent Cells
• Contains F' Episome: No
• Improves Plasmid Quality: Yes (endA1)
• Cloning Methylated DNA: Yes (mcrA)
• Transformation Efficiency Level: Medium Efficiency (1 x 10⁸ to 1 x 10⁹ cfu/μg)
• Content And Storage: • One Shot TOP10/P3 Chemically Competent E. coli (21 x 50 μL)
  Store Competent Cells at –80°C.
  
  • pUC19 Plasmid (50 μL)
  Store pUC19 plasmid at –20°C.
  
  S.O.C. Medium (6 mL)
  Store S.O.C. Medium at 4°C or at room temperature
• Antibiotic Resistance Bacterial: Yes (Streptomycin, Kanamycin, Ampicillin, Tetracycline)
• Cloning Unstable DNA: No
• Blue/White Screening: Yes (lacZΔM15)
• High-throughput Compatibility: Low
• Preparing Unmethylated DNA: No
• Reduces Recombination: Yes (recA1)
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): No
• Species: E. coli (K12)
• Format: Tube
• Product Line: One Shot™
• Quantity: 21 x 50 μL

For Research Use Only. Not for use in diagnostic procedures.