Invitrogen™ PCR_X Enhancer System

PCR_x Enhancer System is used in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates.

Supplier:  Invitrogen™ 11495017

Catalog No. 11-495-017

Includes: PCR_x Enhancer Solution consists of 10X PCR_x Amplification Buffer, 10X PCR_x Enhancer Solution, and 50mM magnesium sulfate.

Description

PCR_X Enhancer System is an optimized buffer and cosolvent system designed for use in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates. 10X PCRX Enhancer Solution with 10X PCR_X Amplification Buffer offers higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of DNA polymerases such as Taq DNA polymerase and Platinum Taq DNA Polymerase.

Conventional PCR buffer components can be directly substituted with the 10X PCR_X amplification buffer and 50 mM MgSO₄ for a more robust amplification and improved product yield in some cases.

Notes

Optimal concentration of 10X PCRX Enhancer Solution will vary depending on GC content, Mg++ concentration, and annealing temperature.

Order Info

Shipping Condition: Dry Ice

Specifications

• Content And Storage: • 10X PCRx Enhancer Solution (2 x 1 mL)
  • 10X PCRx Amplification Buffer (2 x 1 mL)
  • 50 mM MgSO₄ (1 x 1 mL)
  
  Store at -20°C.
• Quantity: 250 rxns
• For Use With (Application): Standard PCR, Hot-start PCR
• Reaction Format: Separate Components

• Polymerase: Platinum Taq DNA Polymerase, Platinum Taq DNA Polymerase High Fidelity, Taq DNA Polymerase
• Shipping Condition: Dry Ice
• No. of Reactions: 250 Reactions
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.