Invitrogen™ PCR SuperMix

Ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification

Supplier:  Invitrogen™ 10572014

Catalog No. 10-572-014

Description

Simply add template and primers, reducing set-up time by half. PCR SuperMix contains Mg²⁺, dNTPs, and recombinant Taq DNA Polymerase at concentrations sufficient for routine PCR of fragments up to 5kb. PCR SuperMix is supplied at a 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions.

ChIP-on-Chip, Chromatin Biology, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR

Specifications

• Content And Storage: Contains 4 vials (1.125 ml each) of PCR SuperMix.
  
  Components:
  22 mM Tris-HCl (pH 8.4), 55 mM KCl, 1.65 mM Magnesium Chloride, 220 µM dGTP, 220 µM dATP, 220 µM dTTP, 220 µM dCTP, 22 U/ml recombinant Taq DNA Polymerase, and stabilizers.
• Detection Method: Primer-Probe
• Format: SuperMix or Master Mix
• Hot Start: No
• Overhang: 3'-A
• Reaction Format: SuperMix or Master Mix
• Size (Final Product): 5 kb or less
• Exonuclease Activity: 5' - 3'
• Quantity: 100 Reactions
• Sufficient For: 100 Reactions

• Concentration: 1.1X
• Fidelity (vs. Taq): 1 X
• GC-Rich PCR Performance: Low
• No. of Reactions: 100 Reactions
• Polymerase: Taq DNA Polymerase
• Reaction Speed: Standard
• Starting Material: DNA
• Product Type: PCR SuperMix
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• For Use With (Application): PCR Amplification