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Invitrogen™ Platinum™ Taq DNA Polymerase Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase

Manufacturer:  Invitrogen™ 10966083

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Catalog No. 10-966-083


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Description

Description

Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR. The “hot start” property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step.

  • Convenience: assemble PCR reactions at room temperature
  • Specificity: “hot start” kinetics reduce nonspecific primer annealing, improving product yield
  • Product size: use for PCR products up to 10kb
  • Flexibility: optional KB Extender gives more versatility in PCR assays

Using PlatinumTaq DNA Polymerase

  • Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'3' exonuclease activity (but not 3'5' exonuclease activity)
  • PCR products generated with PlatinumTaq DNA Polymerase may be used in the same downstream applications without protocol modifications

Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Fast PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Noncoding RNA Research, Routine PCR

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Specifications

Specifications

Each box contains:
• 1,000 µL Platinum® Taq DNA Polymerase
• 50 mL 10X PCR Buffer (no MgCl2)
• 10 mL 50 mM MgCl2
• 10 mL KB Extender
Store at -30°C to -10°C in a non-frost-free freezer.
1 X
Built-In Hot Start
Platinum® Taq DNA Polymerase
5000 reactions
Standard
5 kb or less
5' - 3'
Low
3'-A
Platinum®
Separate Components
Approved for shipment on Wet or Dry Ice
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For Research Use Only. Not for use in diagnostic procedures.