Invitrogen™ Platinum™ Taq DNA Polymerase

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

Supplier:  Invitrogen™ 10966083

Catalog No. 10-966-083

Includes: Each box contains: Platinum Taq DNA Polymerase; 10X PCR Buffer (no magnesium chloride); 50mM magnesium chloride; KB Extender

Description

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable hot-start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.

The hot-start property allows for convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity. PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications.

Features

• Convenient reaction assembly at room temperature
• 5' to 3' exonuclease activity
• Lacks 3' to 5' exonuclease activity

Applications

• Amplification of DNA from complex genomic, viral, and plasmid templates
• Hot-start PCR
• RT-PCR

Notes

• For superior PCR performance, the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase is recommended.
• Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

Specifications

• Content And Storage: • Platinum Taq DNA Polymerase, 1000 μL
  • 10X PCR Buffer (no magnesium), 50 mL
  • 50 mM MgCl₂, 10 mL
  • KB Extender, 10 mL
  
  Store at -10°C to -30°C in a non-frost-free freezer.
• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Product Type: DNA Polymerase
• Shipping Condition: Dry Ice
• Fidelity (vs. Taq): 1X
• No. of Reactions: 5000 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

• Format: Tube
• Polymerase: Platinum Taq DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Quantity: 5,000 reactions
• For Use With (Application): Hot-start PCR
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.