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Invitrogen™ Platinum™ Taq DNA Polymerase High Fidelity Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Ideal for amplification of DNA fragments when high yields and robust amplification are required

Manufacturer:  Invitrogen™ 11304029

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Catalog No. 11-304-029


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Description

Description

High fidelity is provided by a mixture of Platinum Taq DNA Polymerase and the proofreading (3´5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum automatic “hot-start” technology.

  • Fidelity: greater than six times higher fidelity than Taq DNA polymerase
  • Amplicon size: amplification of fragments up to 15 kb
  • Convenience: room temperature reaction assembly
  • Applications: amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR
Unit definition
  • One unit of Platinum™ Taq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C

Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), High Fidelity PCR, Hot Start PCR, Long Fragment PCR, Methylation Analysis, PCR, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research, Sanger Sequencing, Sanger Sequencing Technology & Accessories, Sequencing

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Specifications

Specifications

• 1 x 100 µL Platinum Taq DNA Polymerase High Fidelity (5 U/µL)
• 1 x 2.25 mL 10X High Fidelity Buffer [600 mM Tris-SO4 (pH 8.9), 180 mM (NH4)2SO4]
• 1 x 1 mL 50 mM MgSO4

Store at -10°C to -30°C.
6 X
Built-In Hot Start
Platinum® Taq DNA Polymerase High Fidelity
500 reactions
Standard
20 kb or less
3' - 5', 5' - 3'
Low
Mixed
Platinum®
Separate Components
Dry Ice
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For Research Use Only. Not for use in diagnostic procedures.