Invitrogen™ Platinum™ Taq DNA Polymerase, high fidelity

Platinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.

Supplier:  Invitrogen™ 11304102

Catalog No. 11-304-102

Includes: Platinum Taq DNA Polymerase High Fidelity (1000μL, at 5U/μL) is supplied with a bottle (50mL) of 10X High Fidelity Buffer (600mM Tris-SO₄ (pH 8.9), 180mM (NH₄)₂SO₄), and a bottle (25mL) of 50mM MgSO₄.

Description

Platinum™ Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum™ Taq DNA Polymerase and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum™ automatic 'hot-start' technology. Features of Platinum™ Taq DNA Polymerase, High Fidelity:

• Fidelity—greater than six times higher fidelity than Taq DNA polymerase
• Amplicon size—amplification of fragments up to 15 kb (see figure)
• Convenience—room temperature reaction assembly
• Applications—amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR

Unit definition
One unit of Platinum™ Taq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage: • Platinum Taq DNA Polymerase High Fidelity (1 x 1000 μL at 5 U/μL)
  • 10X High Fidelity Buffer (1 x 50 mL)
  • 50 mM MgSO₄ (1 x 25 mL)
  
  Store at -10°C to -30°C.
• GC-Rich PCR Performance: Low
• Polymerase: Platinum Taq DNA Polymerase High Fidelity
• Product Type: DNA Polymerase
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Hot Start: Built-In Hot Start
• Overhang: Mixed, 3'-A
• Size (Final Product): 20 kb or less

• Detection Method: Primer-probe
• PCR Method: 1-step RT-qPCR
• Reaction Speed: Standard
• Shipping Condition: Dry Ice
• Fidelity (vs. Taq): 6X
• No. of Reactions: 5000 Reactions
• Reaction Format: Separate Components

For Research Use Only. Not for use in diagnostic procedures.