Invitrogen™ Platinum™ Taq DNA Polymerase High Fidelity  

Ideal for amplification of DNA fragments when high yields and robust amplification are required

Manufacturer:  Invitrogen™ 11304102

Catalog No. 11-304-102

Includes: Platinum Taq DNA Polymerase High Fidelity (1000µL, at 5U/µL) is supplied with a bottle (50mL) of 10X High Fidelity Buffer (600mM Tris-SO₄ (pH 8.9), 180mM (NH₄)₂SO₄), and a bottle (25mL) of 50mM MgSO₄.

Description

High fidelity is provided by a mixture of PlatinumTaq DNA Polymerase and the proofreading (3'-5' exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum automatic “hot-start” technology.

• Fidelity: greater than six times higher fidelity than Taq DNA polymerase
• Amplicon size: amplification of fragments up to 15kb
• Convenience: room temperature reaction assembly
• Applications: amplification of DNA from complex genomic, viral, and plasmid templates and RT-PCR

Unit definition:
One unit of PlatinumTaq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.

High Fidelity PCR, Hot Start PCR, Long Fragment PCR, PCR, PCR and Real-Time PCR, Sanger Sequencing, Sanger Sequencing Technology and Accessories, Sequencing

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Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage: • 1 x 1000 µL Platinum Taq DNA Polymerase High Fidelity (5 U/µL)
  • 1 x 50 mL 10X High Fidelity Buffer [600 mM Tris-SO₄ (pH 8.9), 180 mM (NH₄)2SO₄]
  • 1 x 25 mL 50 mM MgSO₄
  
  Store at -10°C to -30°C.
• Hot Start: Built-In Hot Start
• Shipping Condition: Dry Ice
• For Use With (Application): PCR Amplification
• Polymerase: Platinum® Taq DNA Polymerase High Fidelity
• Sufficient For: 5000 Reactions

• Exonuclease Activity: 3' - 5', 5' - 3'
• Product Line: Platinum®
• Detection Method: Primer-Probe
• PCR Method: RT-PCR
• Reaction Speed: Standard

For Research Use Only. Not for use in diagnostic procedures.