Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Supplier:  Invitrogen™ 11754100

Catalog No. 11-754-100

Description

The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Streamline Your Workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.

Ideal for Your qRT-PCR Application
CellsDirect technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps. The CellsDirect One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.

CellsDirect One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan™) or LUX™ fluorogenic primers.

• Efficiency: Offers significant time and cost savings for increased throughput
• Convenience: Enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
• Sensitivity: No separate RNA isolation step increases sensitivity by minimizing sample loss
• Broad Detection Range: Detect targets from samples ranging from a single cell to 10,000 cells
• Versatility: Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
• For use with ABI 7000, 7300, 7700, and 7900(HT) instruments

This kit includes 2X Reaction Mix containing ROX™ Reference Dye. An equivalent kit containing a separate tube of ROX Reference Dye, is also available.

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage: • Resuspension Buffer, 10 mL
  • Lysis Enhancer, 1 mL
  • 1 U/μL DNase I, Amplification Grade, 500 μL
  • 10X DNase I Buffer, 160 μL
  • 25 mM EDTA, 400 μL
  • SuperScript™ III RT/Platinum™ Taq Mix, 100 μL
  • 2X Reaction Mix with ROX™, 2 x 1.25 mL
  • 50 mM MgSO₄, 1 mL
  • DEPC-treated water, 2 mL
  • 10 ng/μL HeLa Total RNA, 10 μL
  
  The kit is shipped on dry ice and should be stored at -20°C.
• Reaction Speed: Fast or Standard
• Product Line: CellsDirect™, Platinum™, SuperScript™
• Concentration: 2X
• Sample Type: Cells

• Detection Method: Primer-probe
• For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
• For Use With (Application): RT-PCR
• No. of Reactions: 100 Reactions

For Research Use Only. Not for use in diagnostic procedures.