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Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Convenient 2X format allows further addition of template and primer at any desired concentration

Manufacturer:  Invitrogen™ 10928042

Encompass

Catalog No. 10-928-042


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Description

Description

  • The SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient, sensitive, and reproducible detection and analysis of RNA molecules by RT-PCR
  • Components for both cDNA synthesis and PCR are combined in a single tube, using gene-specific primers and target RNAs from either total RNA or mRNA
  • Reverse transcription automatically follows PCR cycling without additional steps
  • Using the SuperScript One-Step System The system consists of two major components: RT/Platinum Taq Mix and 2X Reaction Mix
  • The RT/Platinum Taq Mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase for optimal cDNA synthesis and PCR amplification
  • SuperScript II RT is a modified version of Moloney Murine Leukemia Virus (M-MLV) RT, engineered to reduce RNase H activity and increase thermal stability
  • Platinum Taq DNA polymerase is Taq DNA polymerase complexed with a proprietary antibody that inhibits polymerase activity at ambient temperatures
  • The antibody is denatured and polymerase activity is restored during the denaturation step in PCR cycling at 94°C
  • This provides an automatic “hot start” in PCR, increasing sensitivity, specificity, and yield
  • The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers

  • Final product size: 3kb or less

One-Step RT-PCR, PCR & Real-Time PCR, RT-PCR, Reverse Transcription

Specifications

Specifications

SuperScript™ One-Step RT-PCR System components are listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored.
Gel Electrophoresis
Kit
Built-In Hot Start
1-Step RT-PCR
Master Mix
Reverse Transcription
3 kb or Less
1-Step RT-PCR
100μL SuperScript II RT/Platinum Taq DNA Polymerase mix; 3 x 1mL 2X reaction mix; 500μL 5mM magnesium sulfate; 1mL 50mM magnesium sulfate
Unlabeled Oligos
One-Step RT-PCR System
SuperScript™ II
100 Reactions
2 X
2 X
High
100 Reactions
Platinum Taq
Standard
RNA
RNA
1 mL
100 Reactions
Platinum™, SuperScript™
100 Reactions
Wet Ice
SDS
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