Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase

Description

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR. This system combines RT/Platinum Taq mix and 2X reaction mix. The RT/Platinum Taq mix contains a mixture of SuperScript II Reverse Transcriptase and Platinum Taq DNA Polymerase. The 2X reaction mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg²⁺ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers.

All components for cDNA synthesis and PCR are combined in a single tube with gene-specific primers and target RNA. Reverse transcription automatically follows PCR cycling without additional steps.

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System (Cat. No. 12594025) or SuperScript IV One-Step RT-PCR System with ezDNase (Cat. No. 12595025). The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

Specifications

• Content And Storage:

  • SuperScript II RT/Platinum Taq DNA Polymerase mix (100 μL)
  • 2X Reaction mix (3 x 1 mL)
  • 5 mM magnesium sulfate (500 μL)
  • 50 mM magnesium sulfate (1 mL)
  
  Store at –20°C.
  
  

• Detection Method: Gel Electrophoresis
• Format: Kit
• PCR Method: 1-step RT-PCR
• Polymerase: Platinum Taq DNA Polymerase
• Reaction Speed: 30 to 50 min.
• Technique: 1-step RT-PCR
• Optimal Reaction Temperature: 55°C to 60°C
• Quantity: 100 rxns
• Reverse Transcriptase: SuperScript II
• Ribonuclease H Activity: Reduced
• Shipping Condition: Wet Ice
• Final Product Type: PCR Amplified cDNA
• Hot Start: Built-In Hot Start
• No. of Reactions: 100 Reactions
• Reaction Format: Master Mix
• Reagent Type: Reverse Transcription
• Size (Final Product): Up to 7 kb
• Starting Material: RNA

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.