Invitrogen™ Human IFN gamma ProQuantum Immunoassay Kit

Description

ELISA

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Specifications

• Accession Number: P01579
• Assay Range: 0.0128 to 5000 pg/mL
• Assay Sensitivity: <0.01 pg/mL
• Conjugate: FAM™
• Detection Method: Taqman™ Real-time PCR
• Product Type: ProQuantum
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Cytokine Protein Quantitation
• For Use With (Equipment): qPCR Instruments
• Gene ID (Entrez): 3458
• Gene Symbol: IFG; IFI
• Kit Contents: Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
• Label or Dye: FAM™
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
• Storage Requirements: -20°C
• Target: IFN gamma
• Test Time: 30 mins
• Total Assay Time: 2 hrs

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov