Invitrogen™ Human IL-8 ProQuantum Immunoassay Kit

Description

ELISA

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

Specifications

• Accession Number: P10145
• Assay Range: 0.0128 to 5000 pg/mL
• Assay Sensitivity: <0.01 pg/mL
• Conjugate: FAM™
• Detection Method: Taqman™ Real-time PCR
• Product Type: ProQuantum
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): Cytokine Protein Quantitation
• For Use With (Equipment): qPCR Instruments
• Gene ID (Entrez): 3576
• Gene Symbol: GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1
• Kit Contents: Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
• Label or Dye: FAM™
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
• Storage Requirements: -20°C
• Target: IL-8
• Test Time: 30 mins
• Total Assay Time: 2 hrs

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov