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Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade

Catalog No. 25530049
Encompass_Preferred
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Quantity:
5 mL

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

  • Over a wide pH range—optimal activity between 6.5 and 9.5
  • Under denaturing conditions—e.g., in the presence of SDS or urea
  • In the presence of metal chelating agents—e.g., EDTA
  • At comparatively high temperatures—optimum digestion temperature is 65°C
  • Protein Filter: P

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmol of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

TRUSTED_SUSTAINABILITY

Specifications

Concentration 20 mg/mL
Purity DNase-Free, RNase-Free
For Use With (Application) Chromatin Biology/RNA Extraction/DNA Extraction
Content And Storage Store in freezer (-5 to -30°C).
Shipping Condition Approved for shipment on Wet or Dry Ice
Quantity 5 mL
Product Type Proteinase K
Form Liquid

For Research Use Only. Not for use in diagnostic procedures.

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