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Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade
Description
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
- Over a wide pH range—optimal activity between 6.5 and 9.5
- Under denaturing conditions—e.g., in the presence of SDS or urea
- In the presence of metal chelating agents—e.g., EDTA
- At comparatively high temperatures—optimum digestion temperature is 65°C
- Protein Filter: P
Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmol of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.
Specifications
Specifications
| Concentration | 20 mg/mL |
| Purity | DNase-Free, RNase-Free |
| For Use With (Application) | Chromatin Biology/RNA Extraction/DNA Extraction |
| Content And Storage | Store in freezer (-5 to -30°C). |
| Shipping Condition | Approved for shipment on Wet or Dry Ice |
| Quantity | 5 mL |
| Product Type | Proteinase K |
| Form | Liquid |
For Research Use Only. Not for use in diagnostic procedures.
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