Learn More
Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade

Description
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
- Over a wide pH range—optimal activity between 6.5 and 9.5
- Under denaturing conditions—e.g., in the presence of SDS or urea
- In the presence of metal chelating agents—e.g., EDTA
- At comparatively high temperatures—optimum digestion temperature is 65°C
- Protein Filter: P
Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmol of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Specifications
Specifications
Concentration | 20 mg/mL |
Purity | DNase-Free, RNase-Free |
For Use With (Application) | Chromatin Biology/RNA Extraction/DNA Extraction |
Content And Storage | Store in freezer (-5 to -30°C). |
Shipping Condition | Approved for shipment on Wet or Dry Ice |
Quantity | 5 mL |
Product Type | Proteinase K |
Form | Liquid |
For Research Use Only. Not for use in diagnostic procedures.
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.