Invitrogen™ Proteinase K Solution

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.

Supplier:  Invitrogen™ 4333793

Catalog No. 43-337-93

Description

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.

• Over a wide pH range (optimal activity between 6.5 and 9.5)
• Under denaturing conditions (e.g., the presence of SDS or urea)
• In the presence of metal chelating agents (e.g. EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and Quality Testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit Definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Order Info

Shipping Condition: Wet Ice

Specifications

• Purity: DNase-Free, RNase-Free
• Content And Storage: Store in refrigerator (2°C to 8°C).
• Quantity: 5 mL
• Form: Liquid

• For Use With (Application): Chromatin Biology/RNA Extraction/DNA Extraction
• Shipping Condition: Wet Ice
• Product Type: Proteinase K

For Research Use Only. Not for use in diagnostic procedures.