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Invitrogen™ Proteinase K Solution
Description
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.
- Over a wide pH range (optimal activity between 6.5 and 9.5)
- Under denaturing conditions (e.g., the presence of SDS or urea)
- In the presence of metal chelating agents (e.g. EDTA)
- At comparatively high temperatures (optimum digestion temperature is 65°C)
Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and Quality Testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit Definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.
Order Info
Shipping Condition: Wet Ice
Specifications
Specifications
| Purity | DNase-Free, RNase-Free |
| For Use With (Application) | Chromatin Biology/RNA Extraction/DNA Extraction |
| Content And Storage | Store in refrigerator (2°C to 8°C). |
| Shipping Condition | Wet Ice |
| Quantity | 5 mL |
| Product Type | Proteinase K |
| Form | Liquid |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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