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Invitrogen™ Proteinase K Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Inactivate endogenous nucleases when isolating RNA or DNA

Manufacturer:  Invitrogen™ 4333793

Catalog No. 43-337-93


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Description

Description

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.

  • Over a wide pH range (optimal activity between 6.5 and 9.5)
  • Under denaturing conditions (e.g., the presence of SDS or urea)
  • In the presence of metal chelating agents (e.g. EDTA)
  • At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications:
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and Quality Testing:
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit Definition:
One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

DNA and RNA Purification and Analysis, DNA Extraction, General RNA Purification Reagents and Accessories, General gDNA Purification Reagents and Accessories, Genomic DNA Purification, RNA Extraction

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Shipping Condition: Wet Ice

Specifications

Specifications

5mL, 5mL
Proteinase K Solution, 5mL, 2 to 8°C Storage, Removal of Endogenous Nucleases During the Preparation of DNA and RNA, Preparation of Tissue Sections for in Situ Hybridization
DNA and RNA purification and analysis, RNA extraction, DNA extraction, genomic DNA purification, general RNA purification reagents and accessories, general gDNA purification reagents and accessories. Removal of endogenous nucleases during the preparation
Proteinase K Solution
Store in refrigerator (2° to 8°C).
Proteinase K Solution
Liquid
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For Research Use Only. Not for use in diagnostic procedures.