Invitrogen™ PureLink™ Quick Gel Extraction Kit

Catalog No.	Quantity
K210025	250 Preps
K210012	50 Preps

Catalog No. K210025 Supplier Invitrogen™ Supplier No. K210025

Description

Includes

Gel Solubilization Buffer (L3)
Wash Buffer (W1)
Elution Buffer (E5); (10 mM Tris-HCl, pH 8.5)
Quick Gel Extraction Columns
Wash Tubes
Recovery Tubes

The PureLink™ Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages.

The PureLink™ Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages. DNA can be extracted and purified from agarose gels with different melting points in ∼30 minutes using PureLink™ silica membrane-based quick gel extraction columns. For your convenience, purification protocols are provided for centrifugation and for vacuum.

Advantages of using PureLink™ Quick Gel Extraction Kit:

• Purify DNA fragments from TAE and TBE agarose gels of various percentages and melting points
• Complete the procedure in ∼30 minutes
• Easily purify DNA fragments from 40 bp to 10 kb from gels
• Obtain high recovery of DNA fragments
• Bind and purify up to 15 μg DNA with one column
• Purify DNA fragments that are high quality and show reliable performance in PCR, restriction enzyme digestion, cloning, and labeling

Note: The PureLink™ Quick Gel Extraction Kit is not designed to purify supercoiled plasmid DNA or genomic DNA from agarose gels. Only linear DNA fragments may be purified from gels using this kit.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage:

  All components of the PureLink™ Quick Gel Extraction System are shipped at room temperature. Upon receipt, store all components at room temperature.
  For better long-term performance store purification columns at 2°C to 8°C.
  Components:
  • Gel Solubilization Buffer (L3)
  • Wash Buffer (W1)
  • Elution Buffer (E5); (10 mM Tris-HCl, pH 8.5)
  • Quick Gel Extraction Columns
  • Wash Tubes
  • Recovery Tubes

• Format: Kit
• Isolation Technology: Spin or Vacuum Column, Silica Spin Column
• Sample Type: DNA Fragments in Agarose Gel Slices, Gel Samples
• For Use With (Application): Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, In Vitro Transcription
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Label or Dye: Ethidium Bromide, SYBR Safe
• Product Line: PureLink
• Product Type: Quick Gel Extraction Kit
• Quantity: 250 Preps
• Shipping Condition: Room Temperature
• Target: Nucleic Acids in Gel Fragments
• Test Time: 30 min.

Frequently Asked Questions (FAQs)

My downstream enzymatic reactions are not working after purifying my DNA with your PureLink Quick Gel Extraction Kit. What should I do?

Here are some suggestions for your experiments:

- Many enzymes, including restriction endonucleases and ligases, are inhibited by small amounts of agarose and by perchlorate contaminants. This is not normally a problem. If it is, however, you can use the DNA without repurification by increasing the amount of enzyme used in the digest or ligation or by increasing the digestion incubation time. Also, you may use less DNA in your digest or ligation with the same total volume and the same amount of restriction enzyme.
- There may have been residual ethanol in the eluted fragment. Be sure to thoroughly centrifuge to remove the wash buffer, discard the wash buffer, and use a fresh tube to collect the eluted DNA. For applications that are very sensitive to ethanol, let the open spin column stand for 15 minutes at room temperature to let any excess ethanol evaporate.
- The washing steps may not be as efficient as they should be. Under these circumstances, there may be trace amounts of perchlorate in the eluate. To avoid this, extend the centrifugation times to 5 minutes and wash 2 times with wash buffer.
- Be sure to perform the optional wash step if you are using higher concentrations of agarose or are adding more than 250 mg to the cartridge. If applications are sensitive to EDTA, elute with water (pH 7.5-8.5), or with 10 mM Tris, pH 8.0 without EDTA.

Can I use water to elute my sample using the PureLink Quick Gel Extraction Kit?

Yes, water may be used, but please ensure that the water is clean and the pH of the water is between 7.5 and 8.5.

I'm running an agarose gel with TAE or TBE. Can I use your gel extraction kits?

Yes, both TAE and TBE agarose gels are compatible with our gel extraction kits.

What is the difference between Purelink PureLink Quick Gel Extraction System and S.N.A.P. Gel Purification Kit?

The PureLink kit can be used with both TAE and TBE gels, while the S.N.A.P. Gel Purification Kit is recommended for TAE gels only.

Can you use TBE gels with S.N.A.P. gel purification kits?

No, TBE gels contain borate, which interferes with the sodium iodide solution. Therefore, only TAE gels can be used. We recommend the PureLink Quick Gel Extraction kit for both TAE and TBE agarose gels.

What is the maximum amount of DNA that can be bound and purified per column using the PureLink Quick Gel Extraction Kit (Cat. No. K210012, K210025)?

One column from the PureLink Quick Gel Extraction Kit (Cat. No. K210012, K210025) can bind and purify up to 15 µg DNA.

What is unique about the S.N.A.P. UV-free Gel Purification Kit?

The S.N.A.P. UV-free Gel Purification Kit utilizes crystal violet as a staining reagent, which can be visualized in non-UV light conditions. This can prevent DNA damage that can be caused by the UV light required by normal ethidium bromide staining. DNA fragments recovered using this kit have higher cloning efficiency. In addition, DNA can be visualized under normal light as a thin violet band while the gel is running and excised as soon as bands are sufficiently resolved.

For Research Use Only. Not for use in diagnostic procedures.