Invitrogen™ Ribonuclease H

Description

Includes

Ribonuclease H containing 30 units supplied at a concentration of 2U/μL

Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA

• Source: Purified from E. coli expressing the E. coli RNase H gene on a plasmid
• Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease
• Unit definition: One unit hydrolyzes 1nmol of RNA in ³H-labeled poly(A)poly(dT) to acid-soluble material in 20 min. at 37°C
• Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl₂ , 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol ³H-labeled poly(A)poly(dT), and enzyme in 50μL for 20 min. at 37°C

Removal of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription

Order Info

Shipping Conditions: Approved for shipment on wet or dry ice

Specifications

• Concentration: 2 U/μL
• Content And Storage: 4 vials of Ribonuclease H with each containing 30 units supplied at a concentration of 2U/μl
  
  Store at -20°C in a non-frost-free freezer.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Enzyme: RNase
• Quantity: 120 U
• Product Type: Ribonuclease H

For Research Use Only. Not for use in diagnostic procedures.