Invitrogen™ Ribonuclease H

Catalog No.	Quantity
18-021-071	120 U
18-021-014	30 U

Catalog No. 18-021-071 Supplier Invitrogen™ Supplier No. 18021071

Description

Includes

Ribonuclease H containing 30 units supplied at a concentration of 2U/μL

Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA

• Source: Purified from E. coli expressing the E. coli RNase H gene on a plasmid
• Performance and quality testing: Ribonuclease, nonspecific endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease
• Unit definition: One unit hydrolyzes 1nmol of RNA in ³H-labeled poly(A)poly(dT) to acid-soluble material in 20 min. at 37°C
• Unit reaction conditions: 20mM Tris-HCl (pH 7.5), 0.1M KCl, 10mM MgCl₂ , 0.1mM DTT, 5% (w/v) sucrose, 0.5nmol ³H-labeled poly(A)poly(dT), and enzyme in 50μL for 20 min. at 37°C

Removal of mRNA during second-strand cDNA synthesis; Removal of poly(A) sequences from mRNA in the presence of oligo(dT); Oligodeoxyribonucleotide-directed cleavage of RNA; PCR & Real-Time PCR; Reverse Transcription

Order Info

Shipping Conditions: Approved for shipment on wet or dry ice

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Specifications

• Concentration: 2 U/μL
• Content And Storage: 4 vials of Ribonuclease H with each containing 30 units supplied at a concentration of 2U/μl
  
  Store at -20°C in a non-frost-free freezer.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Enzyme: RNase
• Quantity: 120 U
• Product Type: Ribonuclease H

Frequently Asked Questions (FAQs)

Do you offer RNAse H from the SuperScript III First Strand Synthesis System as a stand-alone item?

Yes, we do offer E. coli RNAse H as a stand-alone item: Cat. Nos. 18021014 and 18021071.

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

For Research Use Only. Not for use in diagnostic procedures.