Invitrogen™ S1 Nuclease  

Single-strand-specific endonuclease hydrolyzes single-stranded RNA or DNA into 5' mononucleotides

Manufacturer:  Invitrogen™ 18001016

Catalog No. 18-001-016

Includes: S1 Nuclease is supplied with a vial of 10X S1 Nuclease buffer [300mM sodium acetate (pH 4.6), 10mM zinc acetate, 50% (v/v) glycerol], vial of dilution buffer, vial of 3M NaCl.

Description

Hydrolyzes single-stranded regions in duplex DNA such as loops and gaps.

• S1 Nuclease is stable at 65°C.
• Applications: Nuclease mapping techniques, Removal of single-stranded regions from double-stranded DNA, Exo III-ordered sequencing
• Source: Isolated from Aspergillus oryzae.
• Performance and Quality Testing: Double-strand-specific deoxyribonuclease and phosphatase assays
• Unit Definition: One unit hydrolyzes 1µg of denatured DNA to acid-soluble material in 1 min. at 37°C
• Unit Reaction Conditions: 30mM sodium acetate (pH 4.6), 50mM NaCl, 1mM zinc acetate, 0.5 mg/mL heat-denatured DNA, 5% (v/v) glycerol, and enzyme in 0.5mL for 10 min. at 37°C

Cloning, DNA and RNA Purification and Analysis, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis and Blotting, Restriction Enzyme Cloning

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Order Info

Shipping Condition: Approved for shipment on Wet or Dry Ice

Specifications

• Product Type: Nuclease, S1
• Includes: S1 Nuclease is supplied with a vial of 10X S1 Nuclease buffer [300mM sodium acetate (pH 4.6), 10mM zinc acetate, 50% (v/v) glycerol], vial of dilution buffer, vial of 3 M NaCl
• For Use With (Application): Cloning, DNA and RNA Purification and Analysis, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis and Blotting, Restriction Enzyme Cloning
• Quantity: 20,000 units

For Research Use Only. Not for use in diagnostic procedures.