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Invitrogen™ Subcloning Efficiency™ DH5α Competent Cells

Catalog No. 18265017
Encompass_Preferred
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Quantity:
4 x 500 μL

Includes

Subcloning Efficiency DH5α Competent Cells: 4 vials, 500μL each (total of 2mL), pUC19 DNA (100pg/μL): 1 vial, 20μL

Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA.

Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA. Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any general application where DNA for transformation is not limiting.

Features of Subcloning Efficiency DH5α Competent Cells
• Designed for general, low throughput, everyday use
• Standard laboratory strain useful for general cloning applications

Ideal for routine subcloning procedures
Subcloning Efficiency DH5α Competent Cells are recommended for routine subcloning of genes into plasmid vectors. 500 μL of chemically competent cells are pre-aliquoted in tubes for ten reactions using 50 μL. These cells yield >1 x 106 transformants/μg control DNA per 50 μL reaction. Subcloning Efficiency DH5α Competent Cells are not suitable for the generation of cDNA libraries.

Flexible cloning capabilities
Benefits of Subcloning Efficiency DH5α Competent Cells include:
lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
recA1 that ensures increased insert stability and prevents unwanted recombination 
endA1 that improves the yield and quality of plasmid DNA prepared from minipreps
• Supports replication of M13mp vectors but not plaque formation

Genotype
F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-

Find the strain and format that you need
• We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs.
• Strains are available for challenging cloning applications such as library preparation or cloning unstable DNA.
• SOC medium (Cat. No. 15544034) for E. coli recovery after transformation is available, as well as other ready-to-use bacterial growth formats.

Order Info

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Product Type Chemically Competent Cells
Contains F' Episome No
Improves Plasmid Quality Yes (endA1)
Cloning Methylated DNA No
Transformation Efficiency Level Subcloning Efficiency (106 to 107 cfu/μg)
Content And Storage • Subcloning Efficiency DH5α Competent Cells (4 x 500 μL)
Store Competent Cells at –80°C.

• pUC19 DNA (20 μL at 100 pg/μL)
Store pUC19 DNA at –20°C.

Antibiotic Resistance Bacterial No
Cloning Unstable DNA Not suitable for cloning unstable DNA
Blue/White Screening Yes (lacZΔM15)
High-throughput Compatibility Low
Preparing Unmethylated DNA No
Reduces Recombination Yes (recA1)
Shipping Condition Dry Ice
T1 Phage - Resistant (tonA) No
Species E. coli (K12)
Format Tube
Product Line Subcloning Efficiency™
Quantity 4 x 500 μL
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For Research Use Only. Not for use in diagnostic procedures.

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