SuperScript™ II Reverse Transcriptase Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

An improved version of SuperScript RT

Manufacturer: Invitrogen 18064014

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Catalog No. 18-064-014

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    Concentration 200U/μL
    Format Frozen
    Includes SuperScript II Reverse Transcriptase (10,000 units total, at 200U/μL), 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and 100mM DTT
    Packaging Type Tube
    Quantity 50 reactions (10,000 units)
    Temperature (English) Operating 107.6deg.F
    Temperature (Metric) Operating 42deg.C
    Test Sensitivity Medium

    SuperScript II Reverse Transcriptase (RT) is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations in the RNase H active center to reduce RNase H activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript II RT superior performance characteristics.

    • Yield: Reduced RNase H activity results in greater first-strand cDNA yields
    • Size of cDNA: Generation of RT-PCR products up to 12kb
    • Thermostability:Full activity at 42°C
    • Flexibility:Uses either total or poly(A)+ RNA
    • Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
    • Concentration: 200 U/µL
    • Unit definition:One unit of SuperScript II RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)·oligo(dT)12-18 as template
    • primer Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)12-18, 0.1mg/mL BSA, and enzyme in 50µL for 10 minutes at 37°C

    • Optimal reaction temperature: 42°C
    • Final product size: 12.3kb or less

    PCR & Real-Time PCR, Reverse Transcription

  • Description & Specifications

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