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Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix

Optimized SuperMix formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA

Manufacturer:  Invitrogen™ 18080400


Catalog No. 18-080-400

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Includes: 2X Reaction Mix (500μL); SuperScript III Enzyme Mix (100μL); Oligo(dT)20 (50μL total provided at 50μM); Random hexamers (50μL total provided at 50ng/μL); Annealing buffer (50μL)



RNA targets from 100 bp to >12kb can be detected with this system. The amount of starting material can vary from 0.1pg to 5μg of total RNA.

  • Kit includes SuperScript™ III/RNaseOUT™ Enzyme Mix, 2X First-Strand Reaction Mix, and Annealing Buffer
  • SuperScript™ III Reverse Transcriptase, included in the Enzyme Mix, is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability
  • Enzyme can be used to synthesize cDNA at a temperature range of 45° to 55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases
  • Because SuperScript™ III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA
  • RNaseOUT™ Recombinant RNase Inhibitor is included in the Enzyme Mix to safeguard against degradation of target RNA due to ribonuclease contamination
  • 2X First-Strand Reaction Mix includes 10mM MgCl2 and 1mM of each dNTP in a buffer formulation that has been optimized for first-strand synthesis of cDNA
  • Annealing Buffer is used in the initial template-primer annealing step
  • Separate tubes of oligo(dT)20 and random hexamers are also provided. cDNA synthesis can be performed using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer

  • Optimal reaction temperature: 50°C
  • Enough material provided for 50 reactions (20μL each)

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice



• 2X Reaction Mix (500 μl)
• SuperScript™ III Enzyme Mix (100 μl)
• Oligo(dT)20 (50 μl total provided at 50 μM)
• Random hexamers (50 μl total provided at 50 ng/μl)
• Annealing buffer (50 μl)

Enough material provided for 50 reactions (20 μl each). Store at -20°C.
cDNA (First-Strand)
Reverse Transcription
50 Reactions
Random Primers, Oligo dT Primers
First-Strand Synthesis Super Mix
Dry Ice
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
2 X
Master Mix
50 Reactions
Master Mix
Reverse Transcription
20 μL
SuperScript™ III
50 Reactions


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