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Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Optimized SuperMix formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA

Manufacturer:  Invitrogen™ 18080400

Catalog No. 18-080-400


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Includes: 2X Reaction Mix (500µL); SuperScript III Enzyme Mix (100µL); Oligo(dT)20 (50µL total provided at 50µM); Random hexamers (50µL total provided at 50ng/µL); Annealing buffer (50µL)

Description

Description

RNA targets from 100 bp to >12kb can be detected with this system. The amount of starting material can vary from 0.1pg to 5µg of total RNA.

  • Kit includes SuperScript™ III/RNaseOUT™ Enzyme Mix, 2X First-Strand Reaction Mix, and Annealing Buffer
  • SuperScript™ III Reverse Transcriptase, included in the Enzyme Mix, is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability
  • Enzyme can be used to synthesize cDNA at a temperature range of 45° to 55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases
  • Because SuperScript™ III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA
  • RNaseOUT™ Recombinant RNase Inhibitor is included in the Enzyme Mix to safeguard against degradation of target RNA due to ribonuclease contamination
  • 2X First-Strand Reaction Mix includes 10mM MgCl2 and 1mM of each dNTP in a buffer formulation that has been optimized for first-strand synthesis of cDNA
  • Annealing Buffer is used in the initial template-primer annealing step
  • Separate tubes of oligo(dT)20 and random hexamers are also provided. cDNA synthesis can be performed using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer

  • Optimal reaction temperature: 50°C
  • Enough material provided for 50 reactions (20µL each)

PCR & Real-Time PCR, Reverse Transcription

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Order Info

Shipping Condition: Dry ice

Specifications

Specifications

cDNA Synthesis, RT-PCR (Endpoint), Real-Time PCR
2X Reaction Mix (500μL); SuperScript III Enzyme Mix (100μL); Oligo(dT)(20) (50μL total provided at 50μM); Random hexamers (50μL total provided at 50ng/μL); Annealing buffer (50μL)
50 reactions
122deg.F
SuperMix or Master Mix
Random Primers, Oligo dT Primers
Store at -20deg.C
50deg.C
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