Invitrogen™ SuperScript™ III First-Strand Synthesis System  

Optimized to synthesize first-strand cDNA from purified poly(A)⁺ or total RNA or RT-PCR

Manufacturer:  Invitrogen™ 18080051

Catalog No. 18-080-051

Includes: Oligo(dT)₂₀ (50µM), 50µL; Random hexamers (50ng/µL), 250µL; 10X RT buffer, 1mL; 0.1M DTT, 250µL; 25mM magnesium chloride, 500µL; 10mM dNTP mix, 250µL; SuperScript III RT (200U/µL), 50µL; RNaseOUT (40U/µL), 100µL; E. coli RNase H (2U/µL), 50µL; DEPC-treated water, 1.2mL; Total HeLa RNA (10ng/µL), 20µL; Sense Control Primer (10µM), 25µL; Antisense Control Primer (10µM), 25µL

Description

RNA targets from 100 bp to >12kb can be detected with this system. The amount of starting material can vary from 1pg to 5µg of total RNA. SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42° to 55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it may be used to synthesize first-strand cDNA from a total RNA preparation.

• Using the SuperScript III First-Strand System: cDNA synthesis is performed in the first step using either total RNA or poly(A)⁺-selected RNA primed with oligo(dT), random primers, or a gene-specific primer
• In the second step, PCR is performed in a separate tube using primers specific for the gene of interest
• For the PCR reaction, one of the following DNA polymerases is recommended
• Platinum Taq DNA Polymerase provides automatic hot-start conditions for increased specificity up to 4kb
• Platinum Taq DNA Polymerase High Fidelity provides increased yield and high fidelity for targets up to 15kb
• Platinum Pfx DNA Polymerase provides maximum fidelity for targets up to 12kb

• Optimal reaction temperature: 50°C
• Sufficient for 50 reactions, based on a 20µL reaction size

PCR & Real-Time PCR, Reverse Transcription

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Order Info

Shipping Condition: Dry ice

Specifications

• For Use With (Application): First-Strand cDNA Synthesis, Microarry analysis, RT-PCR
• Quantity: 50 reactions
• Temperature (English) Operating: 122°F

• Includes: Oligo(dT)₍₂₀₎(50μM), 50μL; Random hexamers (50ng/μL), 250μL; 10X RT buffer, 1mL; 0.1M DTT, 250μL; 25mM magnesium chloride, 500μL; 10mM dNTP mix, 250μL; SuperScript III RT (200U/μL), 50μL; RNaseOUT (40U/μL), 100μL; E. coliRNase H (2U/μL), 50μL; DEPC-treated water, 1.2mL; Total HeLa RNA (10ng/μL), 20μL; Sense Control Primer (10μM), 25μL; Antisense Control Primer (10μM), 25μL
• Storage Requirements: Store at -20deg.C
• Temperature (Metric) Operating: 50°C

For Research Use Only. Not for use in diagnostic procedures.