Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ SuperScript™ III First-Strand Synthesis System Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA or RT-PCR

Manufacturer:  Invitrogen™ 18080051

Catalog No. 18-080-051


Add to cart

Includes: Oligo(dT)20 (50µM), 50µL; Random hexamers (50ng/µL), 250µL; 10X RT buffer, 1mL; 0.1M DTT, 250µL; 25mM magnesium chloride, 500µL; 10mM dNTP mix, 250µL; SuperScript III RT (200U/µL), 50µL; RNaseOUT (40U/µL), 100µL; E. coli RNase H (2U/µL), 50µL; DEPC-treated water, 1.2mL; Total HeLa RNA (10ng/µL), 20µL; Sense Control Primer (10µM), 25µL; Antisense Control Primer (10µM), 25µL

Description

Description

RNA targets from 100 bp to >12kb can be detected with this system. The amount of starting material can vary from 1pg to 5µg of total RNA. SuperScript III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42° to 55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it may be used to synthesize first-strand cDNA from a total RNA preparation.

  • Using the SuperScript III First-Strand System: cDNA synthesis is performed in the first step using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer
  • In the second step, PCR is performed in a separate tube using primers specific for the gene of interest
  • For the PCR reaction, one of the following DNA polymerases is recommended
  • Platinum Taq DNA Polymerase provides automatic hot-start conditions for increased specificity up to 4kb
  • Platinum Taq DNA Polymerase High Fidelity provides increased yield and high fidelity for targets up to 15kb
  • Platinum Pfx DNA Polymerase provides maximum fidelity for targets up to 12kb

  • Optimal reaction temperature: 50°C
  • Sufficient for 50 reactions, based on a 20µL reaction size

PCR & Real-Time PCR, Reverse Transcription

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Order Info

Shipping Condition: Dry ice

Specifications

Specifications

First-Strand cDNA Synthesis, Microarry analysis, RT-PCR
50 reactions
122°F
Oligo(dT)(20)(50μM), 50μL; Random hexamers (50ng/μL), 250μL; 10X RT buffer, 1mL; 0.1M DTT, 250μL; 25mM magnesium chloride, 500μL; 10mM dNTP mix, 250μL; SuperScript III RT (200U/μL), 50μL; RNaseOUT (40U/μL), 100μL; E. coliRNase H (2U/μL), 50μL; DEPC-treated water, 1.2mL; Total HeLa RNA (10ng/μL), 20μL; Sense Control Primer (10μM), 25μL; Antisense Control Primer (10μM), 25μL
Store at -20deg.C
50°C
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.