Invitrogen™ T4 DNA Ligase (5 U/μL)

Description

Includes

T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250mM Tris-HCl (pH 7.6), 50mM MgCl₂, 5mM ATP, 5mM DTT, 25% (w/v) polyethylene glycol-8000].

Catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini

The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.

Unit Definition: One unit catalyzes the exchange of 1nmol ³²P-labeled pyrophosphate into ATP in 20 min. at 37°C. (One unit is equal to approximately 300 cohesive-end ligation units.)

Unit Reaction Conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl₂ , 10mM DTT, 66μM ATP, 3.3μM ³²P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min. at 37°C.

Order Info

Shipping Condition: Dry Ice

Specifications

• Concentration: 5 U/μL
• Content And Storage: T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl₂ , 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.
• Shipping Condition: Dry Ice
• Enzyme: Ligase
• Compatible Buffer: 5X Reaction Buffer
• Quantity: 250 U
• Product Type: T4 DNA Ligase

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.