Invitrogen™ Terminal Deoxynucleotidyl Transferase, recombinant  

Performance and quality tested

Manufacturer:  Invitrogen™ 10533073

Catalog No. 10-533-073

Description

Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is DNA polymerase that catalyzes addition of deoxynucleotides to 3fi hydroxyl terminus of DNA.

• Source: Purified from baculovirus clone of calf thymus TdT
• Performance and Quality Testing: Endonuclease, 3' and 5' exodeoxyribonuclease, and levels of incorporation tested
• Unit Definition: One unit incorporates 1nmol dATP into acid-precipitable material in 1 hr. at 37°C, using d(pA)₅₀ as primer
• Unit Reaction Conditions: 0.2M potassium cacodylate (pH 7.2), 10mM MgO₄ C₄ H₆ , 1mM 2-mercaptoethanol, 0.5mg/mL BSA, 100 flM d(pA)₅₀, 1mM [³H]dATP, and enzyme in 0.15mL for 1 h at 37°C

Cloning; Restriction Enzyme Cloning; Homopolymer tailing of vector and insert for cloning; Labeling oligonucleotides with biotin, ³²P- or ³⁵S-label; Apoptosis (TUNEL)

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Specifications

• Quantity: 3 x 500U
• Content And Storage: Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is supplied with vial of 5X buffer [500 mM potassium cacodylate (pH 7.2), 10 mM CoCl₂ , 1 mM DTT]. Store at -20°C.
• Enzyme: Terminal Deoxynucleotidyl Transferase

For Research Use Only. Not for use in diagnostic procedures.