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Invitrogen™ Topoisomerase I

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Quantity:
500 U
Description
- Topoisomerase I is active in the presence of EDTA
- Source: Purified from calf thymus
- Performance and Quality Testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease, and phosphatase assays; conversion of super-coiled DNA to relaxed DNA
- Unit Definition: One unit catalyzes the conversion of 0.5μg of superhelical ΦX174 RF DNA to a relaxed state in 30 min. at 37°C
- Unit Reaction Conditions: 50mM Tris-HCl (pH 7.5), 50mM KCl, 10mM MgCl2 , 0.1mM EDTA, 0.5mM DTT, 30 μg/mL BSA, 0.5μg ΦX174 RF DNA, and enzyme in 50μL for 30 min. at 37°C
Relaxing positively and negatively supercoiled DNA, Producing DNA topoisomers, Cloning, Restriction Enzyme Cloning
Order Info
Shipping Conditions: Wet ice

Specifications
Specifications
Content And Storage | Store in freezer (-5 to -30°C). |
Shipping Condition | Wet Ice |
Enzyme | Topoisomerase |
Quantity | 500 U |
Product Type | Topoisomerase I |
Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
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