Learn More
Invitrogen™ Involucrin Polyclonal Antibody
Rabbit Polyclonal Antibody
Supplier: Invitrogen™ PA137934
Description
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skin tissue.
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.
Specifications
Involucrin | |
Polyclonal | |
Unconjugated | |
IVL | |
1110019C06Rik; involucrin; IVL | |
Rabbit | |
Antigen affinity chromatography | |
RUO | |
3713 | |
-20°C, Avoid Freeze/Thaw Cycles | |
Liquid |
Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry | |
0.267 mg/mL | |
PBS with 1% BSA and 0.1% sodium azide; pH 7.6 | |
P07476 | |
IVL | |
Recombinant protein encoding aa 420-578 of human involucrin. | |
1 mL | |
Primary | |
Human | |
Antibody | |
IgG |
Safety and Handling
Your input is important to us. Please complete this form to provide feedback related to the content on this product.