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Invitrogen™ Involucrin Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA137934

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Catalog No. 01-673-601


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Description

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skin tissue.

Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix. Its gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.
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Specifications

Specifications

Involucrin
Polyclonal
Unconjugated
IVL
1110019C06Rik; involucrin; IVL
Rabbit
Antigen affinity chromatography
RUO
3713
-20°C, Avoid Freeze/Thaw Cycles
Liquid
Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
0.267 mg/mL
PBS with 1% BSA and 0.1% sodium azide; pH 7.6
P07476
IVL
Recombinant protein encoding aa 420-578 of human involucrin.
1 mL
Primary
Human
Antibody
IgG
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