IRF8 Monoclonal Antibody (V3GYWCH), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-3231	Invitrogen™ 17985280	25 μg
50-112-3232	Invitrogen™ 17985282	100 μg

Description

Description: The V3GYWCH monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP). IRF8 is a 50 kDa transcription factor that plays a critical role in the development of dendritic cells (DC). Specifically, IRF8 is essential in the development and differentiation of plasmacytoid DC (pDC) and CD8a+ DC. IRF8-/- mice are deficient in both pDC and CD8a+ DC populations. CD8a- DC are present in normal numbers in IRF8-/- mice but fail to mature upon Toll-like receptor signaling and are functionally impaired in response to in vivo microbial stimulation. IRF8 deficiency does not affect the frequency or viability of DC precursors and as a result retroviral IRF8 transduction restores pDC development from DC progenitors in IRF8-/- mice. Applications Reported: This V3GYWCH antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This V3GYWCH antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One-step protocol: intracellular (nuclear) proteins located under the Resources Tab online. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

IRF8 Monoclonal antibody specifically detects IRF8 in Human, Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: IRF8
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: AI893568; H-ICSBP; Icsbp; Icsbp1; IMD32A; IMD32B; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; Irf8; IRF-8; Myls; transcription factor ICSBP
• Gene Symbols: Irf8
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Irf8

• Clone: V3GYWCH
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Mouse
• Target Species: Human, Mouse
• Gene Accession No.: P23611, Q02556
• Gene ID (Entrez): 15900, 3394
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.