IRS1 Rabbit anti-Human, Mouse, Polyclonal, Bethyl Laboratories

Rabbit Polyclonal Antibody

Supplier:  Bethyl Laboratories, Inc A301158A

Catalog No. 50-155-9404

Description

The recommended shelf life for this product is 1 year from date of receipt. Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

Specifications

• Antigen: IRS1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: IRS1
• Gene Alias: Phospho-Insulin Receptor Substrate-1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16367, 3667
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 0.2 mg/ml
• Formulation: TBS with 0.1% BSA and 0.09% sodium azide
• Gene Accession No.: P35568, P35569
• Gene Symbols: IRS1
• Immunogen: Between 1192 and 1242
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG