Isopropyl-β-D-Thiogalactopyranoside, MP Biomedicals™

Description

Commonly used as a reagent in cloning procedures. Isopropyl-β-D-Thiogalactopyranoside, MP Biomedicals (IPTG) is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosida

IPTG is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal in blue-white color selection of recombinant bacterial colonies.

Specifications

Chemical Identifiers

• CAS: 367-93-1
• Molecular Formula: C9H18O5S
• Molecular Weight (g/mol): 238.30
• MDL Number: MFCD00063273
• InChI Key: BPHPUYQFMNQIOC-NXRLNHOXSA-N
• Synonym: iptg, isopropyl-beta-d-thiogalactopyranoside, isopropyl-beta-d-thiogalactoside, isopropyl thiogalactoside, isopropyl beta-d-thiogalactopyranoside, isopropyl beta-d-thiogalactoside, isopropyl beta-d-1-thiogalactopyranoside, unii-x73vv2246b, 2r,3r,4s,5r,6s-2-hydroxymethyl-6-isopropylthio tetrahydro-2h-pyran-3,4,5-triol, isopropyl-1-beta-d-thiogalactoside
• PubChem CID: 656894
• ChEBI: CHEBI:61448
• IUPAC Name: (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(propan-2-ylsulfanyl)oxane-3,4,5-triol
• SMILES: CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

• Melting Point: 105°C to 115°C
• pH: 5.0 to 7.0
• Quantity: 250 mg
• Solubility Information: Soluble in methanol and water (50mg/mL, clear, colorless solution).
• Optical Rotation: −26° to −33° (c=1, water)
• Formula Weight: 238.3
• Percent Purity: ≥99%
• Concentration or Composition (by Analyte or Components): Working Concentration: The final concentration typically used in indicator plates is 0.2 mM.
• Physical Form: Powder

Safety and Handling

Recommended Storage

0°C, desiccate

WARNING: Reproductive Harm - www.P65Warnings.ca.gov