Isopropyl-β-D-thiogalactopyranoside, MP Biomedicals™

Description

Isopropyl-β-D-thiogalactopyranoside Genomic Grade, >99%, White Powder

• IPTG is a galactose analogue not recognized by β-galactosidase.
• It used as a Lac operon inducer in bacteria. At 1mM concentration, IPTG induces the β-galactosidase gene expression in bacteria, then it allows the selection of Lac+ recombinant bacteria in X-Gal containing medium.
• It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli.
• IPTG (Isopropyl-β-D-thiogalactopyranoside) is a galactose analogue not recognized by β-galactosidase.
• IPTG is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli.

Specifications

Chemical Identifiers

• CAS: 367-93-1
• Molecular Formula: C9H18O5S
• Molecular Weight (g/mol): 238.30
• MDL Number: MFCD00063273
• InChI Key: BPHPUYQFMNQIOC-NXRLNHOXSA-N
• Synonym: IPTG, Isopropyl β-D-1-thiogalactopyranoside
• IUPAC Name: (2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(propan-2-ylsulfanyl)oxane-3,4,5-triol
• SMILES: CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

• Melting Point: 110°C (literature)
• Color: White
• pH: 7
• Quantity: 1 g
• Beilstein: 4631
• Solubility Information: 10g/L in water.
• Optical Rotation: -28.9° to -32° (c=1, water)
• Grade: >99%
• Physical Form: Powder

Safety and Handling

storageNote1

0°C