Janelia Fluor 585, SE

Description

Orange dye (Reactive group: NHS ester). Applications: super resolution microscopy, confocal, two-photon excitation and live cell imaging

Orange fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag™ and SNAP-tag™. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 585 nm; emission maximum = 609 nm; Quantum yield = 0.78; Max extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0990. Exhibits low non-specific background staining.

To measure the absorbance spectrum of this dye we recommend the following solvent: trifluoroethanol plus 0.1% TFA.

We also offer Janelia Fluor™ conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Specifications

• Chemical Name or Material: 1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-7-(3,3-difluoro-1-azetidinyl)-9,9-dimethyl-2(9 H)-anthracenylidene]-3,3-difluoroazetidinium, inner salt
• CAS: 1811539-88-4
• Quantity: 2 mg
• Target: Amine-Reactive Janelia Fluor™ Dyes
• Molecular Formula: C₃₄H₂₇F₄N₃O₆
• Formula Weight: Observed MW: 649.6
• Purity: 95%