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Invitrogen™ Multispecies JNK1/JNK2 (Total/Phospho) InstantOne™ ELISA Kit

Catalog No. 501125035
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50-112-5035 96 Tests
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Catalog No. 50-112-5035 Supplier Invitrogen™ Supplier No. 858619811
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Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of total and phosphorylated human and mouse JNK 1/2 in cell lysates. Detection of rat JNK 1/2 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P45983,P45984,Q91Y86,Q9WTU6
Conjugate HRP
Product Type ELISA
Sample Type Cell Lysates
Target Species Human, Mouse
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 26419, 26420, 5599, 5601
Gene Symbol JNK-55,JNK2,JNK2A,JNK2ALPHA,JNK2B,JNK2BETA,PRKM9,SAPK,SAPK1a,p54a,p54aSAPK,JNK,JNK-46,JNK1,JNK1A2,JNK21B1/2,PRKM8,SAPK1,SAPK1c
Kit Contents Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym Jun N-terminal Kinase
Target JNK1/JNK2
Test Time 1 hrs 20 mins
Total Assay Time 1 hrs 10 mins
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