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Invitrogen™ Jump-In™ CHO-K1 Kit Non-distribution product as customer accommodation.

Jump-In™ CHO-K1 Kit

Manufacturer:  Invitrogen™ A14148

Catalog No. A14148


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Description

Description

The Jump-In™ CHO-K1 Kit allows the targeted integration of genetic material into a specific pre-engineered R4 site in the Jump-In™ CHO-K1 cell line, to create an isogenic stable cell line with less effort and in less time than traditional cell engineering methods.

The high retargeting efficiency, made possible by the R4 sites in the CHO-K1 cell line, allows the use of the isogenic pool for additional experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for the easy selection of a positive stable clone expressing your gene of interest.

The Jump-In™ CHO-K1 Kit Lets You:

  • Quickly and efficiently develop stably engineered isogenic cell pools in about half the time compared to traditional cell engineering methods
  • Utilize isogenic expression from a defined genomic locus as the ideal solution for comparative analysis of gene families, isoforms, or orthologs
  • Generate multiple cell lines in parallel using the simplified work flow
  • Easily access the technology without complicated licenses or restrictions to interpret

    Save Time with Rapid and Efficient Generation of Engineered Cell Lines
    With the Jump-In™ CHO-K1 Kit you can generate functional cell pools in as little as 2 weeks without laborious clone isolation and analysis, and the streamlined protocol makes it easier to generate several cell lines at the same time. Even generation of clonal cell lines can be done with reduced time and effort due to the high percentage of positive clones. In addition, the Jump-In™ technology gives you the freedom to generate an unlimited number of cell lines without restrictive licensing requirements.

    Expand Your Experimental Capabilities
    The Jump-In™ CHO-K1 Kit is the ideal solution for cells and assays where transient engineering technologies are problematic, as well as for difficult to The kit also provides a convenient way to create target panels of gene families, isoforms, or orthologs. Genes coding for large proteins or multi-unit proteins are not a problem since the Gateway™ destination vectors accept large inserts.

    The kit includes:
  • pJTI™ R4 Dest CMV pA vector (100µg)
  • pJTI™ R4 Int vector (100µg)
  • Jump-In™ CHO-K1 Cells (2 vials @ 1 ml each)

    For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
  • Specifications

    Specifications

    Jump-In™ CHO-K1 Kit, 1 Kit, Liquid, Transfection Delivery Method, Mammalian Expression, Jump-In™ CHO-K1 Cell Line, CMV Promoter, Hygromycin Selection Agent, Jump-In™ System, Used in Protein Expression
    1 Kit(s)
    Protein biology, protein expression, mammalian expression, targeted integration, stable cell line development
    Jump-In™ CHO-K1 Kit
    Liquid
    Jump-In™ CHO-K1 cells, pJTI™ R4 Int (integrase vector), pJTI™ R4 DEST CMV pA (destination vector)
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    For Research Use Only. Not for use in diagnostic procedures.