K-562 (human chronic myelogenous leukemia) Whole cell lysate, Non-denatured, Abnova

Non-denatured whole cell lysate of K-562 (human chronic myelogenous leukemia)

Supplier:  Abnova Corporation L009V2

Catalog No. 89-014-367

Description

• Tissue: Bone Marrow
• Host: Human
• Lysis Buffer: Modified RIPA Lysis Buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage buffer: In modified RIPA Lysis Buffer

Western Blotting, Immunoprecipitation

Specifications

• Tissue: Bone Marrow
• Description: Whole cell lysate (non-denatured)
• Preparation Method: Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
• Quantity: 200 μg
• Host Species: Human
• Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.

• For Use With (Application): Immunoprecipitation, Western Blot
• Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
• Storage Buffer: In modified RIPA Lysis Buffer.
• Concentration: 5 mg/mL