Thermo Scientific Chemicals Kanamycin, 50 mg/ml in distilled water, sterile-filtered

Description

Kanamycin monosulfate, CAS # 25389-94-0, is an aminoglycoside sulfate salt with antibacterial activity. | CAS: 25389-94-0 | C18H38N4O15S | 582.575 g/mol

It has been used in tissue culture media. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. It can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100mg/mL. Kanamycin can be used as a selection agent in several types of isolation media.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• Kanamycin monosulfate is the sulfate salt form of kanamycin A
• It was isolated from Streptomyces kanamyceticus
• This compound inhibits translocation during protein biosynthesis. It binds to the 30S ribosomal subunit, causing misreading of the mRNA sequence

Application

• Kanamycin monosulfate shows antibacterial activity. It is effective against gram-positive and gram-negative bacteria, as well as Mycoplasma species
• This compound is also involved in translating prokaryotic ribosomes
• In molecular biology experiments, it is commonly used to select and isolate transformants expressing the kanamycin resistance gene, and plants that have the NPT II (APH3) gene incorporated

Specifications

Chemical Identifiers

• CAS: 25389-94-0
• Molecular Formula: C18H38N4O15S
• Molecular Weight (g/mol): 582.575
• MDL Number: MFCD00070289
• InChI Key: OOYGSFOGFJDDHP-IZQIRFRQSA-N
• PubChem CID: 134129479
• IUPAC Name: (2R,3S,4S,5S,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-diamino-3-[(2S,3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxyoxane-3,4,5-triol;sulfuric acid
• SMILES: C1C(C(C(C(C1N)OC2C(C(C(C(O2)CN)O)O)O)O)OC3C(C(C(C(O3)CO)O)N)O)N.OS(=O)(=O)O

• Type: Kanamycin
• Physical Form: Liquid
• Quantity: 50 mL
• Solubility Information: Fully miscible in water. Insoluble in acetone,chloroform,ether,ethyl acetate,and alcohol.
• Chemical Name or Material: Kanamycin, 50mg/mL in distilled water, sterile-filtered

Safety and Handling

Hazard Category	H317-H334-H360FD
Hazard Statement	GHS H Statement H334-H360-H317 May cause allergy or asthma symptoms or breathing difficulties if inhaled. May damage fertility or the unborn child. May cause an allergic skin reaction.
Precautionary Statement	P201-P202-P261-P272-P280g-P281-P285-P302+P352-P304+P341-P308+P313-P333+P313-P342+P311-P363-P501c
TSCA	No
storageNote1	-30° to -10°C
Recommended Storage	Store at -20°C

SDS

RUO – Research Use Only