Invitrogen™ KCNMB1 Polyclonal Antibody

Catalog No.	Quantity
PA1924	100 μg

Catalog No. PA1924 Supplier Invitrogen™ Supplier No. PA1924

Description

Rabbit Polyclonal Antibody

PA1-924 detects large conductance, calcium-activated potassium channel beta (Maxi K+ Beta) from human, mouse, and rat samples. PA1-924 has been successfully used in western blot and immunofluorescence. By western blot, this antibody detects an ~28kDa protein representing Maxi K+ beta from mouse myometrial smooth muscle cell extracts. The molecular weight detected varies due to glycosylation, multimerization and binding to actin. Immunizing peptide corresponds to amino acid residues 90-103 from human Maxi K+ beta. This sequence is completely conserved across many species. PA1-924 immunizing peptide (Cat. # PEP-119) is available for use in neutralization and control experiments.

Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open. Maxi K+ beta is the target for possible therapeutics because of its role in blood flow and blood pressure regulation.

Specifications

• Antigen: KCNMB1
• Applications: Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene: KCNMB1
• Gene Accession No.: P97678, Q16558, Q8CAE3
• Gene Alias: big potassium channel beta subunit 1; BK channel beta subunit; BK channel beta subunit 1; BK channel subunit beta-1; BKbeta; BKbeta1; calcium-activated potassium channel beta subunit; calcium-activated potassium channel subunit beta; Calcium-activated potassium channel subunit beta-1; calcium-activated potassium channel, subfamily M subunit beta-1; Charybdotoxin receptor subunit beta-1; hbeta1; hslo-beta; K(VCA)beta; k(VCA)beta-1; Kcnmb1; large conductance Ca2+-activated K+ channel beta 1 subunit; maxi K channel subunit beta-1; MaxiK channel beta-subunit 1; potassium calcium-activated channel subfamily M regulatory beta subunit 1; potassium channel subfamily M regulatory beta subunit 1; potassium large conductance calcium-activated channel, subfamily M, beta member 1; slo-beta; slo-beta-1; slowpoke-beta
• Gene Symbols: KCNMB1
• Host Species: Rabbit
• Immunogen: Synthetic Peptide: L(90) Y H T E D T R D Q N Q Q C(103).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 16533, 29747, 3779
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG