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Sigma Aldrich Fine Chemicals Biosciences KiCqStartR SYBRR Green qPC
Supplier: Sigma Aldrich Fine Chemicals Biosciences KCQS01250RXN
KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated ready-to-use reaction cocktail that contains all components except primers and template for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer stabilizers and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency sensitivity specificity and robust fluorescent signal using fast or conventional cycling protocols with SYBR Green qPCR.Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye an antibody-mediated Hot-Start Taq DNA polymerase dNPTs MgCl2 and proprietary buffers and stabilizers.
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