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Alexa Fluor™ Kinase Tracer 199, 25 μM

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Quantity:
25 μL
Description
- Color: Far-Red
- Excitation/Emission (nm): 340/665
- Label or Dye: Alexa Fluor™ 647
- Target Enzyme: Kinases (Multiple)
- Validated Kinases: EGFR T790M L858R, EPHB4, KIT V654A, MAPK9 (JNK2), RIPK2
- In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 199 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.

Specifications
Specifications
Assay | Kinase Binding Assay |
Assay Entry | Biochemical binding/kinetic |
Color | Far-Red |
Content And Storage | Store at -20°C. |
Detection Method | Fluorescence |
Excitation/Emission | 340/665 |
For Use With (Application) | TR-FRET |
Label or Dye | Alexa Fluor™ 647 |
Quantity | 25 μL |
Shipping Condition | Dry Ice |
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For Research Use Only. Not for use in diagnostic procedures.
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