Invitrogen™ Kinesin 5B Monoclonal Antibody (KN-02)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA119352

Catalog No. 01-676-108

Description

This antibody recognizes heavy chain of conventional kinesin, a protein associated with intracellular vesicles, and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.

Specifications

• Antigen: Kinesin 5B
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 15mM sodium azide; pH 8
• Gene Accession No.: P33176, Q2PQA9, Q61768
• Gene Symbols: KIF5B
• Immunogen: Enriched fraction of porcine brain kinesin.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Pig
• Product Type: Antibody
• Isotype: IgM

• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Clone: KN-02
• Conjugate: Unconjugated
• Gene: KIF5B
• Gene Alias: AL022807; Conventional kinesin heavy chain; EMBL:ABC25059.1}; epididymis secretory protein Li 61; HEL-S-61; Khc; Khcs; KIF5A; KIF5B; kif5b {ECO:0000312; kinesin 1 (110-120kD); kinesin family member 5A; kinesin family member 5B; kinesin heavy chain; kinesin heavy chain member 5B, ubiquitous; kinesin-1 heavy chain; KINH; KNS; Kns1; ubiquitous kinesin heavy chain; UKHC; U-KHC; Unknown (protein for IMAGE:7944745)
• Host Species: Mouse
• Purification Method: Sequential Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 117550, 16573, 3799, 595132
• Content And Storage: 4°C, do not freeze
• Form: Liquid

Safety and Handling

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