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Thermo Scientific™ Klenow Fragment Available on GSA/VA Contract for Federal Government customers only.

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'3' polymerase, 3'5' exonuclease (proofreading) activities, but lacking 5'3' exonuclease activity.

Manufacturer:  Thermo Scientific™ EP0054

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Catalog No. FEREP0054


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Description

Description

Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.

Highlights

  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
  • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers

Applications

  • DNA blunting by fill-in 3'-overhangs
  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA
  • DNA sequencing by the Sanger method
  • Site-specific mutagenesis of DNA with synthetic oligonucleotides
  • Second strand synthesis of cDNA

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Specifications

Specifications

2U/μL
E.coli cells with a cloned fragment of the polA gene
300U
metal chelators, PPi, Pi (at high concentrations) (8)
68kDa monomer
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