KN-62

Catalog No.	Quantity
AAJ62444MCR	1 mg
AAJ62444MA	10 mg

Catalog No. AAJ62444MCR Supplier Thermo Scientific Chemicals Supplier No. J62444MCR

Description

Potently and specifically inhibits calcium/calmodulin kinase II | CAS: 127191-97-3 | C38H35N5O6S2 | 721.85 g/mol

It is used as a potent and selective inhibitor of CaMKII. KN-62 has demonstrated potent and selective inhibition of Ca2+/calmodulin-dependent protein kinase II (CaMKII; Ki=0.9 ?M) which causes cells to arrest in the S phase. In HEK-293 cells expressing type III adenylyl cyclase (III-AC), 10 ?M of KN-62 blocked CaMKII phosphorylation of III-AC, and inhibited hormone-stimulated Ca2+ oscillations. Additionally, KN-62 is a potent non-competitive antagonist at the P2X7 receptor (IC50 = 15 nM).

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
It is used as a potent and selective inhibitor of CaMKII. KN-62 has demonstrated potent and selective inhibition of Ca2+/calmodulin-dependent protein kinase II (CaMKII; Ki=0.9 μM) which causes cells to arrest in the S phase. In HEK-293 cells expressing type III adenylyl cyclase (III-AC), 10 μM of KN-62 blocked CaMKII phosphorylation of III-AC, and inhibited hormone-stimulated Ca2+ oscillations. Additionally, KN-62 is a potent non-competitive antagonist at the P2X7 receptor (IC50 = 15 nM).

Solubility
Soluble in 45% (w/v) aq 2-hydroxypropyl-β-cyclodextrin (0.93 mg/ml), methanol (10 mg/ml), DMSO (12 mg/ml), chloroform (20 mg/ml), ethanol (∼1 mg/ml), 1:2 DMF:PBS (pH 7.2) (∼0.3 mg/ml), and DMF & DMSO (∼30 mg/ml).

Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Recommended storage temperature: -20°C. Keep away from strong oxidizing agents.

Specifications

Chemical Identifiers

• CAS: 127191-97-3
• Molecular Formula: C38H35N5O6S2
• Molecular Weight (g/mol): 721.85
• MDL Number: MFCD00083180
• InChI Key: RJVLFQBBRSMWHX-UHFFFAOYNA-N
• Synonym: s-4-2-n-methylisoquinoline-5-sulfonamido-3-oxo-3-4-phenylpiperazin-1-yl propyl phenyl isoquinoline-5-sulfonate, unii-63hm46xpow, chembl28324, 63hm46xpow, 1-n,o-bis 5-isoquinolinesulfonyl-n-methyl-l-tyrosyl-4-phenylpiperazine, s-5-isoquinolinesulfonic acid 4-2-5-isoquinolinylsulfonyl methylamino-3-oxo-3-4-phenyl-1-piperazinyl propyl phenyl ester, 4-2s-2-5-isoquinolinylsulfonyl methylamino-3-oxo-3-4-phenyl-1-piperazinyl propyl phenyl isoquinolinesulfonic acid ester, 4-2s-2-n-methylisoquinoline-5-sulfonamido-3-oxo-3-4-phenylpiperazin-1-yl propyl phenyl isoquinoline-5-sulfonate, 5-isoquinolinesulfonic acid, 4-2s-2-5-isoquinolinylsulfonyl methylamino-3-oxo-3-4-phenyl-1-piperazinyl propyl phenyl ester
• PubChem CID: 5312126
• IUPAC Name: [4-[(2S)-2-[isoquinolin-5-ylsulfonyl(methyl)amino]-3-oxo-3-(4-phenylpiperazin-1-yl)propyl]phenyl] isoquinoline-5-sulfonate
• SMILES: CN(C(CC1=CC=C(OS(=O)(=O)C2=C3C=CN=CC3=CC=C2)C=C1)C(=O)N1CCN(CC1)C1=CC=CC=C1)S(=O)(=O)C1=C2C=CN=CC2=CC=C1

• Melting Point: 65°C to 67°C
• Color: Yellow
• Linear Formula: NH₂(CH₂)₁₀NH₂
• Quantity: 1 mg
• Solubility Information: Soluble in 45% (w/v) aq 2-hydroxypropyl-β-cyclodextrin (0.93mg/ml),methanol (10mg/ml),DMSO (12mg/ml),chloroform (20mg/ml),ethanol (∽1mg/ml),1:2 DMF:PBS (pH 7.2) (∽0.3mg/ml),and DMF & DMSO (∽30mg/ml).
• Formula Weight: 721.84
• Physical Form: Powder
• Chemical Name or Material: KN-62

Safety and Handling

TSCA	No
Recommended Storage	Store at -20°C

SDS

RUO – Research Use Only