Thermo Scientific™ Lambda Exonuclease (10 U/μL)

Description

Lambda Exonuclease is a highly processive exodeoxyribonuclease that selectively digests the 5'-phosphorylated strand of double-stranded DNA in 5'-3' direction.

Thermo Scientific Lambda Exonuclease is a highly processive 5'≥3' exodeoxyribonuclease. It selectively digests the 5'-phosphorylated strand of double-stranded DNA. The enzyme exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has no activity at nicks and limited activity at gaps in DNA.

Highlights

• Active in Thermo Scientific PCR buffers

Applications

• Generating single-stranded PCR products for use in:
  • DNA sequencing
  • Analysis of DNA single-strand conformation polymorphism (SSCP)
  • Rolling circle amplification
   
• Producing single-stranded DNA from double-stranded DNA fragments
• Cloning of PCR products

Note

Use of this enzyme in certain applications may be covered by patents and may require a license.

Specifications

• Concentration: 10 U/μL
• Enzyme: Exonuclease
• Quantity: 1,000 units
• Product Type: Exonuclease

For Research Use Only. Not for use in diagnostic procedures.