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Thermo Scientific™ Lambda Exonuclease (10 U/µL)
Lambda Exonuclease is a highly processive exodeoxyribonuclease that selectively digests the 5'-phosphorylated strand of double-stranded DNA in 5'-3' direction.
Manufacturer: Thermo Scientific™ EN0561
Description
Thermo Scientific Lambda Exonuclease is a highly processive 5'=>3' exodeoxyribonuclease. It selectively digests the 5'-phosphorylated strand of double-stranded DNA. The enzyme exhibits low activity on single-stranded DNA and non-phosphorylated DNA, and has no activity at nicks and limited activity at gaps in DNA.
Highlights
- Active in Thermo Scientific PCR buffers
Applications
- Generating single-stranded PCR products for use in:
- DNA sequencing
- Analysis of DNA single-strand conformation polymorphism (SSCP)
- Rolling circle amplification
- Producing single-stranded DNA from double-stranded DNA fragments
- Cloning of PCR products
Note
Use of this enzyme in certain applications may be covered by patents and may require a license.
Specifications
| 1000U | |
| Exonuclease |
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