Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. K1679B

Thermo Scientific™ LanthaScreen™ ATF2 (19-106) A549 Cell Line

Catalog No. K1679B
Encompass_Preferred
Change view
Click to view available options
Quantity:
1 Vial
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
K1679B 1 Vial
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. K1679B Supplier Thermo Scientific™ Supplier No. K1679B
Only null left
Add to Cart
Add to Cart
Request a Quote

Description

Contains a fusion protein consisting of GFP and a fragment encoding for aa 19-106 of ATF2 under the control of a CMV promotor

  • The LanthaScreen™ATF2 (19-106) A549 cell line contains a fusion protein consisting of GFP and a fragment encoding for aa 19-106 of ATF2 under the control of a CMV promotor. A549 is a lung carcinoma cell line which shows inducible activation of JNK by number of different ligands, such as TNF and EGF, which leads to the transient phosphorylation of GFP-ATF2 (19-106).
  • This LanthaScreen™cell line therefore allows the analysis of JNK activity using GFP-ATF2 phosphorylation as readout. The GFP-ATF2 (19-106) construct was transfected into A549 cells using Lipofectamine™2000, followed by selection with Blasticidin.
  • This cell line is a clonal population isolated by flow cytometry using GFP fluorescence as sorting marker. This cell line is validated for EC50 and Z' under optimized conditions using TNF as ligand for JNK mediated GFP-ATF2 (19-106) phosphorylation.
  • This cell line has been tested for assay performance under variable experimental conditions, including cell plating density, stimulation time, DMSO tolerance and assay development time.
TRUSTED_SUSTAINABILITY

Specifications

Cell State Dividing Cells
Content And Storage LanthaScreen™ Cellular assays are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Quantity 1 Vial
System Type LanthaScreen™
Druggable Target Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.