LAP (Latency Associated peptide) Monoclonal Antibody (FNLAP), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-3229	Invitrogen™ 17982941	25 Tests
17-982-942	Invitrogen™ 17982942	100 Tests

Description

Description: The FNLAP monoclonal antibody reacts with human latency associated peptide (LAP, pro-TGF beta 1, LAP/TGF beta 1). Many different cells produce TGF beta and it mediates effects on the proliferation, differentiation and function of many cell types. TGF beta is synthesized as a precursor that contains LAP at the N-terminus and mature TGF beta at the C-terminus. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells. It is believed that this surface-expressed LAP is due to the binding of LAP to GARP (LRRC32), which is a transmembrane protein that is also found at high levels on platelets and activated regulatory T cells. Applications Reported: This FNLAP antibody has been reported for use in flow cytometric analysis. Applications Tested: This FNLAP antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (0.5 μg) per test.

LAP (Latency Associated peptide) Monoclonal antibody specifically detects LAP (Latency Associated peptide) in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: LAP (Latency Associated peptide)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: CED; DPD1; LAP; latency-associated peptide; prepro-transforming growth factor beta-1; regulatory protein; TGFB; Tgfb1; Tgfb-1; TGFbeta; TGF-beta 1; TGFbeta1; TGF-beta1; TGF-beta-1; transforming growth factor beta 1; transforming growth factor beta-1; Transforming growth factor beta-1 proprotein; transforming growth factor, beta 1; transforming growth factor-beta 1
• Gene Symbols: TGFB1
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: FNLAP
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P01137
• Gene ID (Entrez): 7040
• Immunogen: E. coli derived hLAP-TGFb, mixed with VB3A9.
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: TGFB1

For Research Use Only.