Lasu1/Ureb1 Rabbit anti-Human, Polyclonal, Bethyl Laboratories

Rabbit Polyclonal Antibody

Supplier:  Bethyl Laboratories, Inc A300486A

Catalog No. 50-155-7739

Description

The recommended shelf life for this product is 1 year from date of receipt. Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.

Specifications

• Antigen: Lasu1/Ureb1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HUWE1
• Gene Alias: RP3-339A18.4, ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MULE, UREB1; ARF binding protein 1; ARF-binding protein 1; BJ-HCC-24 tumor antigen; E3 ubiquitin-protein ligase HUWE1; HECT domain protein LASU1; HECT, UBA and WWE domain-containing protein 1; Mcl-1 ubiquitin ligase E3; URE-B1; URE-binding protein 1; homologous to E6AP carboxyl terminus homologous protein 9; large structure of UREB1; upstream regulatory element-binding protein 1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10075
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1 mg/ml
• Formulation: phosphate, tris citrate with 0.09% sodium azide; pH 7-8
• Gene Accession No.: Q7Z6Z7
• Gene Symbols: HUWE1
• Immunogen: Between 2250 and 2300
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG