Thermo Scientific Chemicals LB Medium, Miller

Description

LB Medium, Miller is a bacterial growth medium used for E. coli cultivation for cloning, DNA plasmid production, and production of recombinant proteins.

LB Medium is used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• LB Medium, Miller is used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates
• This product is formulated to enhance growth of recombinant E. coli strains by providing exogenous growth factors tryptone and yeast, which the E. coli would otherwise have to synthesize
• LB Medium, Miller contains high levels of NaCl to allow for osmotic balance
• It also contains 10g Bacto tryptone, 5g Bacto yeast extract, and 10g NaCl per liter

Application

• LB Medium, Miller is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production, and production of recombinant proteins
• It has been used as a culture medium for Escherichia coli, Vibrio natriegens, Pseudomonas aeruginosa, and Bacillus subtilis strains

Specifications

• Form: Liquid
• Packaging Type: Plastic Bottle
• Quantity: 500 mL

Safety and Handling

SDS

RUO – Research Use Only