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MilliporeSigma™ LC3-II Enrichment Kit (Western Blot)

Catalog No. 1710232 Shop All MilliporeSigma Products
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15 Assays
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17-102-32 15 Assays
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Catalog No. 17-102-32 Supplier MilliporeSigma™ Supplier No. 1710232

Description

Includes

LC3-II Enrichment Component Box

  • Autophagy Reagent A: One vial, lyophilized
  • 10x Enrichment Buffer: One bottle containing 10mL 10x Enrichment Buffer
  • 5x Assay Buffer: One bottle containing 15mL 5x Assay Buffer
  • 10x RIPA Lysis Buffer: One bottle containing 1mL 10x RIPA Lysis Buffer

Western Blot Detection Component Box

  • Protease Inhibitor Cocktail: One vial containing 20 μL of 1000x Protease Inhibitor Cocktail
  • Anti-LC3 antibody: One vial containing 20μL of 1000x anti-LC3 rabbit monoclonal antibody
  • Anti-TOMM22 antibody: One vial containing 20μL of 1000x anti-TOMM22 mouse monoclonal antibody
  • Goat anti-Mouse IgG HRP, Secondary Antibody: One vial containing 30μL of 2000X goat anti-mouse HRP conjugate
  • Goat anti-Rabbit IgG HRP, Secondary Antibody: One vial containing 30μL of 2000X goat anti-rabbit HRP conjugate

Requires

  • Pipettors and tips capable of accurately measuring 1-1000μL
  • Graduated serological pipettes
  • Microcentrifuge tubes
  • Mechanical vortex
  • Rocker
  • Distilled or deionized water
  • 1x Dulbecco's Phosphate Buffered Saline (D-PBS)
  • 1x Tris-buffered saline containing 0.05% Tween-20 (TBST)
  • 3% Non-fat dry milk diluted in TBST (TBST-MILK)
  • Earle's Balanced Salt Solution (EBSS)

Enables sensitive and accurate quantification of autophagosome density by utilizing selective permeabilization procedure that removes cytosolic LC3-I and retains autophagosome-bound LC3-II

  • Enables sensitive and accurate quantification of autophagosome density by utilizing a selective permeabilization procedure that removes cytosolic LC3-I and retains autophagosome-bound LC3-II
  • Reveals quantities of LC3-II, without interference from LC3-I, by Western blotting analysis
  • The kit also includes a lysosomal inhibitor, to prevent degradation of LC3-II that occurs upon fusion of the autophagosome with the lysosome
  • An antibody against a mitochondrial outer membrane protein TOMM22 is included for use as a control for specificity of permeabilization, to ensure that organelles remain intact after treatment
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage The kit may be used for up to 4 months after receipt. Avoid repeated freeze/thaw cycles, and aliquot if necessary
For Use With (Application) Western Blotting
Quantity 15 Assays
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