Invitrogen™ LC3B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA116930

Catalog No. 01-950-171

Description

The reaction with mouse LC3 is weaker than human. The immunogen for this antibody production has 100% sequence homology with rat and zebrafish, 92% with mouse, and 91% with bovine proteins.

LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans, is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). LC3B is associated with microtubule assembly and important is in neurogenesis. Recent studies indicate that LC3B plays a vital role in autophagy, a process that involves the bulk degradation of cytoplasmic component. Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). LC3B is a microtubule-associated protein that mediate the physical interactions between microtubules and components of the cytoskeleton. LC3B may play a role in processes involving cancer, aging, metabolic and neurodegenerative disorders and cardiovascular/pulmonary diseases.

Specifications

• Antigen: LC3B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MAP1LC3B2
• Gene Alias: 1010001C15Rik; Atg8; ATG8E; ATG8F; ATG8G; Autophagy-related protein LC3 B; autophagy-related ubiquitin-like modifier LC3 B; LC3; LC3 beta; LC3A; LC3B; light chain 3; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; Map1alc3; MAP1BLC3; MAP1LC3; MAP1LC3A; MAP1LC3B; MAP1LC3B2; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; microtubule associated protein 1 light chain 3 beta 2; microtubule-associated protein 1 light chain 3; microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3 beta 2; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3 beta 2; Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B-like; Microtubule-associated proteins 1A/1B light chain 3B-like protein; Mpl3; RP11-346K17.1; wu:fb60g11; zbs559; zgc:56434
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100349465, 100359928, 100462754, 100726328, 100769810, 322425, 408001, 427559, 479619, 643246, 64862, 67443, 81631
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ChIP Assay, ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Proximity Ligation Assay, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: A6NCE7, O41515, Q62625, Q9CQV6, Q9GZQ8
• Gene Symbols: MAP1LC3B, MAP1LC3B2
• Immunogen: Synthetic peptide between amino acids 1-100 of the human LC3 protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Rabbit, Monkey, Bovine, Hamster, Pig, Chicken, Bacteria, Guinea Pig, Avian, Zebrafish, Invertebrate
• Product Type: Antibody
• Isotype: IgG