LC3B Polyclonal Antibody, Rockland™

Catalog No.	Quantity
50-199-2337	25 μL
50-199-2336	100 μg

Catalog No. 50-199-2337 Supplier Rockland Immunochemicals Supplier No. 600401MN5S

Description

Rabbit Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity purified antibody is directed against human LC3B. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence.

LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans, is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). LC3B is associated with microtubule assembly and important is in neurogenesis. Recent studies indicate that LC3B plays a vital role in autophagy, a process that involves the bulk degradation of cytoplasmic component. Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). LC3B is a microtubule-associated protein that mediate the physical interactions between microtubules and components of the cytoskeleton. LC3B may play a role in processes involving cancer, aging, metabolic and neurodegenerative disorders and cardiovascular/pulmonary diseases.

Specifications

• Antigen: LC3B
• Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: MAP1LC3B2
• Gene Accession No.: A6NCE7, Q9GZQ8
• Gene Alias: 1010001C15Rik; Atg8; ATG8E; ATG8F; ATG8G; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; BOS_17038; Lc3; LC3 beta; LC3A; LC3b; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1ALC3; MAP1BLC3; Map1lc3; MAP1LC3A; Map1lc3b; MAP1LC3B2; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; microtubule associated protein 1 light chain 3 beta 2; microtubule-associated protein 1 light chain 3; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3 beta 2; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3 beta 2; Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B-like; Microtubule-associated proteins 1A/1B light chain 3B-like protein; Mpl3; RP11-346K17.1; wu:fb60g11; zbs559; zgc:56434
• Gene Symbols: MAP1LC3B, MAP1LC3B2
• Host Species: Rabbit
• Quantity: 25 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 643246, 81631
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid