Invitrogen™ LDHA Polyclonal Antibody

Catalog No.	Quantity
PIPA527406	100 μL

Catalog No. PIPA527406 Supplier Invitrogen™ Supplier No. PA527406

Description

Rabbit Polyclonal Antibody

Recommended positive controls: 293T, A431, HeLa, HepG2, MCF-7, MDA-MB-231, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2. Predicted reactivity: Mouse (92%), Rat (91%), Xenopus laevis (80%), Pig (90%), Rabbit (91%), Chicken (80%), Rhesus Monkey (99%), Chimpanzee (100%), Bovine (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).

Specifications

• Antigen: LDHA
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.37 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 20% glycerol, 1% BSA and 0.025% ProClin 300; pH 7
• Gene: LDHA
• Gene Accession No.: P00338, P04642, P06151, P19629
• Gene Alias: Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; GSD11; HEL-S-133P; l7R2; Lactate Dehydrogenase; lactate dehydrogenase 1, A chain; lactate dehydrogenase 3, C chain, sperm specific; lactate dehydrogenase A; lactate dehydrogenase A4; lactate dehydrogenase A-like; lactate dehydrogenase C; lactate dehydrogenase C variant 3; lactate dehydrogenase M; lactate dehydrogenase-A; lactate dehydrogenase-M; lactate dehydrogenase-M (EC 1.1.1.27); Lactic Dehydrogenase; LDH; LDH muscle subunit; LDH testis subunit; Ldh1; Ldh-1; Ldh3; Ldha; LDH-A; ldha protein; ldha1; LDH-A4 muscle-specific isozyme; glycolytic enzyme; Ldhc; LDH-C; Ldhm; LDH-M; LDH-X; L-lactate dehydrogenase; L-lactate dehydrogenase A chain; L-lactate dehydrogenase C chain; M-LDH; OTTHUMP00000165206; PIG19; proliferation-inducing gene 19; renal carcinoma antigen NY-REN-59
• Gene Symbols: LDHA
• Host Species: Rabbit
• Immunogen: Recombinant protein encompassing a sequence within the center region of human LDHA. The exact sequence is proprietary.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 16828, 24533, 29634, 3939
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG