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ATCC Leishmania mexicana (Biagi) Garnham, GFP-expressing promastigote strain MNYC/BZ/62/M379
Supplier: ATCC PRA416
Leishmania mexicana (Biagi) Garnham is a GFP-expressing promastigote strain (strain designation MNYC/BZ/62/M379) supplied as cryopreserved cells for infectious disease research and assay development. Maintain axenic cultures at 25°C under selective conditions and follow recommended thawing and cryopreservation procedures.
- GFP-expressing promastigotes suitable for visualization and assay work.
- Axenic culture; incubate at 25°C.
- Medium: M199, modified, with 300 μg/mL G418 for selection.
- Storage: -80°C for short-term, vapor phase of liquid nitrogen for long-term.
- Propagation: transfer every 7-14 days; follow recommended thaw and cryopreservation steps.
- Isolation: animal source (Sumichrast's vesper rat), Belize, 1962.
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