Novus Biologicals™ Leptin/OB Antibody Pair [HRP]

Catalog No.	Quantity
NB010116	5 Plates
NB010115	15 Plates

Catalog No. NB010116 Supplier Novus Biologicals™ Supplier No. NBP2795385PLATES

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Leptin/OB.

Specifications

• Assay Range: 7.81 pg/mL
• Assay Sensitivity: 7.81-500 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human Leptin/OB.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: FLJ94114, leptin, leptin (murine obesity homolog), leptin (obesity homolog, mouse), Obese protein, obese, mouse, homolog of, Obesity factor, OBOBS
• Gene ID (Entrez): 3952
• Gene Symbol: LEP
• Host Species: Mouse
• Kit Contents:
  Standard: Each vial contains 172 ng of recombinant Human Leptin/OB.
  Capture Antibody: 0.5 mg/mL of mouse anti-Human Leptin/OB monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of mouse anti-Human Leptin/OB monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 5 Plates
• Regulatory Status: RUO
• Research Discipline: Biologically Active Proteins, Cytokine Research, Diabetes Research, Immunology, Lipid and Metabolism
• Storage Requirements: Storage is content dependent.
• Target: Leptin/OB
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.