Invitrogen™ Leptin Receptor Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1053

Catalog No. PA1053

Description

PA1-053 detects leptin (OB-R) from human, mouse, and rat tissues. PA1-053 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa and ~125 kDa protein representing the short and long forms of OB-R from mouse brain extract. This antibody also detects two smaller, unrelated proteins of ~31 kDa and 35 kDa which are unable to be competed with the peptide in mouse liver extract. The PA1-053 immunogen is a synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R. This sequence is completely conserved in mouse OB-R. PA1-053 immunizing peptide (Cat. # PEP-014) is available for use in neutralization and control experiments.

The leptin (OB-R) receptor is a member of the cytokine receptor superfamily which plays an important role in mammalian body weight homeostasis and energy balance. Ligand-induced activation of the OB-R by the adipose tissue-secreted hormone, leptin, appears to activate downstream signaling events through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. Leptin can homo-dimerize OB-R extracellular domains while downstream signaling events are initiated upon homo-dimerization of OB-R intracellular domains. Several isoforms of the OB-R which differ in length and signaling capabilities have been reported. RNA transcripts encoding the OB-R long form have been found in the choroid plexus and in the hypothalamus which has been proposed as a control center for satiety and energy expenditure. Mutations in either the mouse OB-R (db) or leptin (ob) genes have been shown to result in early-onset obesity. Metabolic abnormalities attributable to these genotypes include hypercorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered CNS activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue.

Specifications

• Antigen: Leptin Receptor
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Lepr
• Gene Alias: B219; CD antigen CD295; CD295; CD295 antigen; db; diabetes; Fa; HuB219; HuB219.2; LEPR; LEP-R; Leprb; LEPRD; LEPROT; leptin receptor; Leptin receptor (fatty); leptin receptor gene-related protein; Modb1; OB receptor; obese-like; obl; Obr; OB-R; OB-RGRP
• Host Species: Rabbit
• Purification Method: Ammonium sulfate precipitation
• Regulatory Status: RUO
• Gene ID (Entrez): 16847, 24536, 3953
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 10 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: P48356, P48357, Q62959
• Gene Symbols: Lepr
• Immunogen: Synthetic peptide corresponding to residues K(577) E I Q W K T H E V F D A K S K S A(594) of the extracellular domain of rat OB-R.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG